Gene name |
SPAC15A10.09c |
Gene ID |
11/F08 |
Gene synonyms/obsolete |
|
Gene product |
sequence orphan;
hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
867 |
ORF length (spliced) |
|
Entry clone length |
867 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
37A:G / 273T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC15A10.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAATGGGGTTCAATCC |
Rev primer name |
SPAC15A10.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGTGGACTGTATCCGGAT |
Amino acid length |
288 |
Molecular weight |
31.4 |
Isoelectric point (calc.) |
4.1 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGFIFFALTI/LPFFGPLFL/LEKNIEILNI |
Localization (YFP) |
Golgi; periphery at
cell tip and site of septum formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |