Gene name |
SPAC19B12.12c |
Gene ID |
12/A03 |
Gene synonyms/obsolete |
yip11; yip1;
yip1-a |
Gene product |
possibly involved in
spliceosomal snRNP biogenesis; structural similarity with SMN
protein; smn1/yab8 interacting protein based on 2 hybrid data
and similar to human SIP1; involved in spliceosomal snRNP
biogenesis; similar to Sp yip12 (paralog); duplicated in Sp;
telomerase associated (implicated); no apparent Sc
ortholog |
Entry clone |
Cloned# |
ORF length (unspliced) |
887 |
ORF length (spliced) |
708 |
Entry clone length |
887 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC19B12.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCTCGAAAAGAAAAAG |
Rev primer name |
SPAC19B12.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGTTTGAAAGAGATCTTTT |
Amino acid length |
235 |
Molecular weight |
26.9 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |