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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC21D10.03c
Gene ID 12/E09
Gene synonyms/obsolete SPBC12C2.12c
Gene product lactoylglutathione lyase
Entry clone Cloned
ORF length (unspliced) 909
ORF length (spliced)
Entry clone length 909
No. of intron 0
Sequence status Finished
Sequence results 21G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC21D10.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTCTACTACTGACAT
Rev primer name SPBC21D10.03.Rv
Rev primer SEQ AGAAAGCTGGGTACTTCTGTTCAATGACCTCA
Amino acid length 302
Molecular weight 34.4
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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