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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC4C3.07
Gene ID 12/E10
Gene synonyms/obsolete
Gene product COP9/signalosome complex (subunit 6); MOV34 domain; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 909
ORF length (spliced)
Entry clone length 909
No. of intron 0
Sequence status Finished
Sequence results 745G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC4C3.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTTGGGGACTAAGCA
Rev primer name SPBC4C3.07.Rv
Rev primer SEQ AGAAAGCTGGGTAGGGAAGCAAATTAAGACGG
Amino acid length 302
Molecular weight 33.2
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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