Gene name |
SPCC338.11c |
Gene ID |
12/F06 |
Gene synonyms/obsolete |
uvi22; rrg1 |
Gene product |
experimentally
characterised; UV-induced protein; transcriptionally regulated
by glucose; regulated post transcriptionally by control of
mRNA stability; deletion mutant results in reduced size;
non-essential; overexpression results in elongated cells of G2
delay; involved in G2/M phase progression; regulated by the
SAPK pathway |
Entry clone |
Cloned |
ORF length (unspliced) |
912 |
ORF length (spliced) |
|
Entry clone length |
912 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
393A:T / 791T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC338.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTTTAGAAGACACGGA |
Rev primer name |
SPCC338.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCCATGGTATTTCCATCTA |
Amino acid length |
303 |
Molecular weight |
34.5 |
Isoelectric point (calc.) |
4.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSNSINALEL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |