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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC824.07
Gene ID 12/F07
Gene synonyms/obsolete
Gene product hydroxyacylglutathione hydrolase
Entry clone Cloned
ORF length (unspliced) 913
ORF length (spliced) 771
Entry clone length 913
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC824.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTTTCATATTACGCC
Rev primer name SPAC824.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAGCGTTTTTCATGTCA
Amino acid length 256
Molecular weight 28.4
Isoelectric point (calc.) 6.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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