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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC61.02
Gene ID 12/H05
Gene synonyms/obsolete spt3
Gene product transcriptional regulator; SAGA complex (Spt-Ada-Gcn5-Acetyltransferase); transcription factor TFIID (18 kD subunit)
Entry clone Cloned
ORF length (unspliced) 924
ORF length (spliced)
Entry clone length 924
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC61.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGAGACTGGCAGAAC
Rev primer name SPCC61.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGATAAGATACACACGAGAT
Amino acid length 307
Molecular weight 35.6
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LEAFRRLQI
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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