| Gene name |
SPAC823.15 |
| Gene ID |
13/A05 |
| Gene synonyms/obsolete |
ppa1 |
| Gene product |
minor serine/threonine
protein phosphatase pp2a-1 catalytic subunit; essential;
required for cell cycle control |
| Entry clone |
Cloned
(Re-cloned) |
| ORF length (unspliced) |
930 |
| ORF length (spliced) |
|
| Entry clone length |
930 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC823.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGTCTCTGGAAAAAT |
| Rev primer name |
SPAC823.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAGAAGTAATCGGGTGTT |
| Amino acid length |
309 |
| Molecular weight |
35.2 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |