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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC29B12.05c
Gene ID 13/A06
Gene synonyms/obsolete
Gene product S-adenosylmethionine-dependent methyltransferase activity
Entry clone Cloned
ORF length (unspliced) 930
ORF length (spliced)
Entry clone length 930
No. of intron 0
Sequence status Finished
Sequence results 487A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC29B12.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGACTACCAAGAATTAC
Rev primer name SPAC29B12.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAATGTCAATGAGTCGTCTA
Amino acid length 309
Molecular weight 35.7
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LERLKPLKI
Localization (YFP) cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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