Gene name |
SPAC17A5.09c |
Gene ID |
13/A08 |
Gene synonyms/obsolete |
|
Gene product |
low similarity to
human protein phosphatase inhibitor 2 and S. cerevisiae
GLC8-Modulator of protein; serine/threonine
phosphatase-involved in control of vacuole fusion-by multiple
alignment; conserved WDE*N.*MK**EP*TP[FY] |
Entry clone |
Cloned |
ORF length (unspliced) |
933 |
ORF length (spliced) |
|
Entry clone length |
933 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC17A5.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGGTTAAATCAATTCT |
Rev primer name |
SPAC17A5.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCATTTTCATTCATATTT |
Amino acid length |
310 |
Molecular weight |
34.9 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |