Gene name |
SPAC4F8.03 |
Gene ID |
13/B01 |
Gene synonyms/obsolete |
SPAC644.01c |
Gene product |
conserved
hypothetical; hypothetical protein; UPF0023 family |
Entry clone |
Cloned |
ORF length (unspliced) |
935 |
ORF length (spliced) |
741 |
Entry clone length |
935 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4F8.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTATTAGTCAACCTGT |
Rev primer name |
SPAC4F8.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTCCTAGCCTCGCTCATG |
Amino acid length |
246 |
Molecular weight |
28 |
Isoelectric point (calc.) |
8.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus;
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |