Gene name |
SPAC18G6.03 |
Gene ID |
13/C02 |
Gene synonyms/obsolete |
ypt3 |
Gene product |
small GTPase; Ypt
subfamily; essential; HEAT repeat; GTP-binding protein;
involved in intracellular protein transport; involved in
exocytosis; involved in cytokinesis; post-translational
modification, gerenylgerenylation @ C-terminal XCC; mutant
(ypt3-i5) displays defects in cytokinesis; mutant (ypt3-i5)
displays defects in cell wall integrity; mutant (ypt3-i5)
displays defects in vacuole fusion; mutant (ypt3-i5)
accumulated aberrant Golgi-like structures; functionally
connected to calcineurin |
Entry clone |
Cloned |
ORF length (unspliced) |
943 |
ORF length (spliced) |
645 |
Entry clone length |
943 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
684T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC18G6.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGTCAAGAGGACGAATA |
Rev primer name |
SPAC18G6.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACAACATTGGGAAGAAGAC |
Amino acid length |
214 |
Molecular weight |
23.8 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |