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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1281.07c
Gene ID 13/C01
Gene synonyms/obsolete
Gene product glutathione S-transferase
Entry clone Cloned
ORF length (unspliced) 942
ORF length (spliced)
Entry clone length 942
No. of intron 0
Sequence status Finished
Sequence results 102C:T / 112A:G / 386T:G / 453T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1281.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCAACACTCATATTAC
Rev primer name SPCC1281.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAATATTCGACATTGGGA
Amino acid length 313
Molecular weight 37
Isoelectric point (calc.) 7.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi?; nucleus>=cytosol; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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