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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC13G7.11
Gene ID 13/C08
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan; confirmed introns (2)
Entry clone Cloned#
ORF length (unspliced) 947
ORF length (spliced) 810
Entry clone length 947
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC13G7.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATGTTCTTAGAACTCA
Rev primer name SPAC13G7.11.Rv
Rev primer SEQ AGAAAGCTGGGTACTCCTTTCCGGAGAAGAGT
Amino acid length 269
Molecular weight 31.6
Isoelectric point (calc.) 10.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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