Gene name |
SPCC790.03 |
Gene ID |
13/C09 |
Gene synonyms/obsolete |
|
Gene product |
rhomboid family
protease; peptidase S54 family; 6 predicted transmembrane
helices; predicted N-terminal signal sequence; probable serine
protease (By similarity) |
Entry clone |
Cloned |
ORF length (unspliced) |
947 |
ORF length (spliced) |
747 |
Entry clone length |
947 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC790.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTCTAGGACGATCAAA |
Rev primer name |
SPCC790.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCCCCCAAGACGGGTTCCT |
Amino acid length |
248 |
Molecular weight |
27.8 |
Isoelectric point (calc.) |
9.6 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
6 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |