Gene name |
SPAC823.13c |
Gene ID |
13/D09 |
Gene synonyms/obsolete |
|
Gene product |
predicted coiled-coil
protein; involved in inner mitochondrial membrane organization
and biogenesis |
Entry clone |
Cloned |
ORF length (unspliced) |
954 |
ORF length (spliced) |
|
Entry clone length |
954 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC823.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGCAACGACTTTCTCA |
Rev primer name |
SPAC823.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACGCTTGAATGCTAAACCA |
Amino acid length |
317 |
Molecular weight |
36.7 |
Isoelectric point (calc.) |
10.1 |
Signal SEQ |
|
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
187 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFVVVQLIL |
Localization (YFP) |
mitochondrion |
Comments for localization |
aggregates |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |