| Gene name |
SPAC644.04 |
| Gene ID |
13/F11 |
| Gene synonyms/obsolete |
pct1 |
| Gene product |
RNA 5'-triphosphatase;
involved in mRNA capping; essential; dimer |
| Entry clone |
Cloned |
| ORF length (unspliced) |
966 |
| ORF length (spliced) |
912 |
| Entry clone length |
966 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
36A:G / 356A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC644.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACCTTAAAGGTTTATT |
| Rev primer name |
SPAC644.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGAATGCTCTCGTCTCAAA |
| Amino acid length |
303 |
| Molecular weight |
35.4 |
| Isoelectric point (calc.) |
6.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |