Gene name |
SPAC17G6.02c |
Gene ID |
13/H11 |
Gene synonyms/obsolete |
|
Gene product |
involved in sphingoid
long-chain base release |
Entry clone |
Cloned |
ORF length (unspliced) |
975 |
ORF length (spliced) |
|
Entry clone length |
975 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
696G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC17G6.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATTACGATAACGCTTT |
Rev primer name |
SPAC17G6.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGCTCCTCAGAGGAGAAA |
Amino acid length |
324 |
Molecular weight |
35.9 |
Isoelectric point (calc.) |
6 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
7 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGQIIALII |
Localization (YFP) |
Golgi?; periphery at
cell tip and site of septum formation; cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |