Gene name |
SPAP27G11.04c |
Gene ID |
14/D08 |
Gene synonyms/obsolete |
|
Gene product |
tRNA-specific
adenosine-34 deaminase subunit |
Entry clone |
Cloned |
ORF length (unspliced) |
991 |
ORF length (spliced) |
948 |
Entry clone length |
991 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAP27G11.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTGAAAACTAATATTTC |
Rev primer name |
SPAP27G11.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGACATGAATATTCTCCTTT |
Amino acid length |
315 |
Molecular weight |
36.2 |
Isoelectric point (calc.) |
8.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
Microscope used for
observation |
DeltaVision |