| Gene name |
SPAC30.02c |
| Gene ID |
14/D09 |
| Gene synonyms/obsolete |
|
| Gene product |
polynucleotide kinase;
RNA polymerase II elongator associated protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
991 |
| ORF length (spliced) |
846 |
| Entry clone length |
991 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
845T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC30.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCATTAATAATTGTTTC |
| Rev primer name |
SPAC30.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTCCAAGGTTTCAAATTGA |
| Amino acid length |
281 |
| Molecular weight |
32.4 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKVTLETCLIL/LTCLFDYLFL/LNIIQGYLDL/LQNCVGYLTL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |