| Gene name |
SPAC25G10.03 |
| Gene ID |
14/E02 |
| Gene synonyms/obsolete |
zip1 |
| Gene product |
transcription factor;
bZIP (basic leucine zipper) transcription factor family;
overexpression suppresses Atf1 deletants |
| Entry clone |
Cloned |
| ORF length (unspliced) |
993 |
| ORF length (spliced) |
|
| Entry clone length |
993 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
208A:G / 281T:C /
935A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC25G10.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTTTACTCCTAATAG |
| Rev primer name |
SPAC25G10.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAGTTAGAAGTAGGACGT |
| Amino acid length |
330 |
| Molecular weight |
36.1 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
multi-septated cells;
elongated cells |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |