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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G8.02
Gene ID 14/E01
Gene synonyms/obsolete
Gene product uridine ribohydrolase; involved in pyrimidine degradation; similar to Sp SPBC1683.06c (paralog)
Entry clone Cloned
ORF length (unspliced) 993
ORF length (spliced)
Entry clone length 993
No. of intron 0
Sequence status Finished
Sequence results 317T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17G8.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTAACACAATTGATTC
Rev primer name SPAC17G8.02.Rv
Rev primer SEQ AGAAAGCTGGGTACAAATAATTGAGCCTGTCT
Amino acid length 330
Molecular weight 36
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; nuclear dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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