Gene name |
SPCC18.07 |
Gene ID |
14/E04 |
Gene synonyms/obsolete |
rpc53 |
Gene product |
DNA directed RNA
polymerase III subunit |
Entry clone |
Cloned |
ORF length (unspliced) |
993 |
ORF length (spliced) |
|
Entry clone length |
993 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC18.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAAAATTTACTCCGAA |
Rev primer name |
SPCC18.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAATCAGGACTAACGATC |
Amino acid length |
330 |
Molecular weight |
36.8 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQSLAQHLGL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |