Gene name |
SPAC823.07 |
Gene ID |
14/E06 |
Gene synonyms/obsolete |
|
Gene product |
Per1 family;
eukaryotic conserved protein; involved in protein processing
in the ER; involved in Mn2+ homeostasis |
Entry clone |
Cloned |
ORF length (unspliced) |
996 |
ORF length (spliced) |
|
Entry clone length |
996 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
374A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC823.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAGTTTTGAGGAACTT |
Rev primer name |
SPAC823.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCGACTGCTATCATAGGCT |
Amino acid length |
331 |
Molecular weight |
38.3 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
8 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi; vacuole
membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |