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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC63.07
Gene ID 14/E11
Gene synonyms/obsolete
Gene product conserved hypothetical; conserved protein
Entry clone Cloned
ORF length (unspliced) 996
ORF length (spliced) 786
Entry clone length 996
No. of intron 3
Sequence status Finished
Sequence results 308T:C / 646T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC63.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCCAAGTCAAGGTTTGA
Rev primer name SPCC63.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTGTAACAATACCTCAAGA
Amino acid length 261
Molecular weight 31
Isoelectric point (calc.) 8.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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