Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC830.10
Gene ID 14/E12
Gene synonyms/obsolete
Gene product involved in the control of 6-N-hydroxylaminopurine sensitivity and mutagenesis
Entry clone Cloned
ORF length (unspliced) 996
ORF length (spliced) 567
Entry clone length 996
No. of intron 6
Sequence status Finished
Sequence results 299A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC830.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACAAGTAAGTCAATTTT
Rev primer name SPCC830.10.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTTTTTTCCGATTCCAAA
Amino acid length 188
Molecular weight 20.9
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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