| Gene name |
SPAC186.02c |
| Gene ID |
14/F04 |
| Gene synonyms/obsolete |
|
| Gene product |
2-hydroxyacid
dehydrogenase; D isomer specific; no apparent Sc ortholog;
similar to Sp SPAC186.07C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
999 |
| ORF length (spliced) |
|
| Entry clone length |
999 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
(-6)T:C / 117T:C /
126A:G / 541A:G / 795A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC186.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGTGTTGTCCTTTTTAG |
| Rev primer name |
SPAC186.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTATTTGCGATTACTGAT |
| Amino acid length |
332 |
| Molecular weight |
36.3 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |