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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1281.04
Gene ID 14/G03
Gene synonyms/obsolete
Gene product aldo/keto reductase; pyridoxal reductase homolog
Entry clone Cloned
ORF length (unspliced) 1002
ORF length (spliced)
Entry clone length 1002
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1281.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTATTGTGAACGGTTT
Rev primer name SPCC1281.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGATGGAAAGGGTGGATTCA
Amino acid length 333
Molecular weight 36.8
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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