Gene name |
SPAC1F3.04c |
Gene ID |
14/G04 |
Gene synonyms/obsolete |
|
Gene product |
conserved protein;
DUF367; putative metal binding domain |
Entry clone |
Cloned |
ORF length (unspliced) |
1003 |
ORF length (spliced) |
867 |
Entry clone length |
1003 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
372A:G / 436A:G /
786A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1F3.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGCCCAAGGAGTTCTAA |
Rev primer name |
SPAC1F3.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGTATCAGTAGAATGAACT |
Amino acid length |
288 |
Molecular weight |
32.6 |
Isoelectric point (calc.) |
5.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGCVRNLRI |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Leica |