Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4G8.11c
Gene ID 14/G05
Gene synonyms/obsolete atp10
Gene product involved in mitochondrial F1-F0 ATPase assembly; ATP synthesis coupled proton transport
Entry clone Cloned
ORF length (unspliced) 1003
ORF length (spliced) 804
Entry clone length 1003
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC4G8.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCATATAATTATTTAAA
Rev primer name SPAC4G8.11.Rv
Rev primer SEQ AGAAAGCTGGGTAACCAACGTTTTTATTTACC
Amino acid length 267
Molecular weight 31.3
Isoelectric point (calc.) 10.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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