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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1709.11c
Gene ID 14/G10
Gene synonyms/obsolete png2
Gene product zinc finger protein; zf-PHD finger; similar to human candidate tumour suppressor p33(ING1) in their C-terminal regions; involved in chromatin mediated transcriptional regulation; involved in transcriptional regulation
Entry clone Cloned
ORF length (unspliced) 1005
ORF length (spliced) 918
Entry clone length 1005
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1709.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGGACGAGTGGAATTGA
Rev primer name SPBC1709.11.Rv
Rev primer SEQ AGAAAGCTGGGTATTGACGACTTTCAGATGAT
Amino acid length 305
Molecular weight 34.8
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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