Gene name |
SPBC651.07 |
Gene ID |
15/C09 |
Gene synonyms/obsolete |
|
Gene product |
COP9/signalosome
associated (pers. comm. Tony Carr); no apparent ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1022 |
ORF length (spliced) |
807 |
Entry clone length |
1022 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
61A:T / 497T:C /
732T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC651.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTGTAATGTACGTTCC |
Rev primer name |
SPBC651.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGAACCATTACTATAGACT |
Amino acid length |
268 |
Molecular weight |
30.1 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVGPIVQTLSI/LVTSVREILEL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |