Gene name |
SPCC757.08 |
Gene ID |
15/E10 |
Gene synonyms/obsolete |
|
Gene product |
exosome (RNase
complex); involved in rRNA processing |
Entry clone |
Cloned |
ORF length (unspliced) |
1029 |
ORF length (spliced) |
876 |
Entry clone length |
1029 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC757.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAGGCATCTGGAAAC |
Rev primer name |
SPCC757.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGACCGCTCGTTCTCTGCC |
Amino acid length |
291 |
Molecular weight |
32.3 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNSLEKGLRL/LSDFRSLEI |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |