Gene name |
SPBC577.11 |
Gene ID |
15/E11 |
Gene synonyms/obsolete |
|
Gene product |
sequence orphan;
hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1030 |
ORF length (spliced) |
720 |
Entry clone length |
1030 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
411A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC577.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAACAAGATCTTTTTGG |
Rev primer name |
SPBC577.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCCTTTACGAGGATACGTT |
Amino acid length |
239 |
Molecular weight |
27.4 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER; Golgi?;
periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |