Gene name |
SPAC22H12.02 |
Gene ID |
15/F12 |
Gene synonyms/obsolete |
|
Gene product |
transcription
initiation factor TFIIF (small subunit); SWI/SNF complex
|
Entry clone |
Cloned |
ORF length (unspliced) |
1036 |
ORF length (spliced) |
726 |
Entry clone length |
1036 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC22H12.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACAACTGTGAAAAGAAC |
Rev primer name |
SPAC22H12.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATAGTGACGCGTTTAGCA |
Amino acid length |
241 |
Molecular weight |
27.6 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYTLPDNLLL |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |