| Gene name |
SPAC1B2.03c |
| Gene ID |
16/A03 |
| Gene synonyms/obsolete |
|
| Gene product |
GNS1/SUR4 family
protein; involved in membrane lipid metabolism; similar to Sp
SPAC1639.01c |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1048 |
| ORF length (spliced) |
1005 |
| Entry clone length |
1048 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
369A:G / 802T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1B2.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATCTAACCGGAGCACA |
| Rev primer name |
SPAC1B2.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGAGTTTCCTTGAGCGAGCA |
| Amino acid length |
334 |
| Molecular weight |
38.3 |
| Isoelectric point (calc.) |
9.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
6 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWHLFEQLSI/LNYLTKYLEL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |