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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC8E11.11
Gene ID 16/E10
Gene synonyms/obsolete SPAC26A3.17c
Gene product putative N-methyltransferase
Entry clone Cloned
ORF length (unspliced) 1074
ORF length (spliced)
Entry clone length 1074
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC8E11.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGACGCTCCACTAGTGAA
Rev primer name SPAC8E11.11.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAATCAAAAGTGACAATG
Amino acid length 357
Molecular weight 40.7
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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