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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC8E11.03c
Gene ID 16/E09
Gene synonyms/obsolete dmc1; dmp1; spdmc1
Gene product involved in meiotic recombination; DNA-binding protein; helix-hairpin-helix; AAA family ATPase; involved in strand exchange
Entry clone Cloned in 2004 trial
ORF length (unspliced) 1073
ORF length (spliced) 999
Entry clone length 1073
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC8E11.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGAATTCGCAGAGGG
Rev primer name SPAC8E11.03.Rv
Rev primer SEQ AGAAAGCTGGGTAGGAAACGTCTGCTATGCCA
Amino acid length 332
Molecular weight 36.4
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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