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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC29A3.13
Gene ID 16/G08
Gene synonyms/obsolete
Gene product conserved hypothetical; PWWP domain protein; involved in cell growth; similar to Sp SPBC215.07C and SPAC23D3.01
Entry clone Cloned
ORF length (unspliced) 1080
ORF length (spliced)
Entry clone length 1080
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC29A3.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATAATGCCCGCACTAA
Rev primer name SPBC29A3.13.Rv
Rev primer SEQ AGAAAGCTGGGTACATGCCTTTTATCGATGGT
Amino acid length 359
Molecular weight 40.9
Isoelectric point (calc.) 9.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 8
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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