Gene name |
SPBC23E6.10c |
Gene ID |
16/G09 |
Gene synonyms/obsolete |
|
Gene product |
translation initiation
factor; eif-2 family; involved in translation |
Entry clone |
Cloned |
ORF length (unspliced) |
1080 |
ORF length (spliced) |
|
Entry clone length |
1080 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
172A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC23E6.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGTTGCAAGCAATCAT |
Rev primer name |
SPBC23E6.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGAAAAATCATACTTATTG |
Amino acid length |
359 |
Molecular weight |
39.3 |
Isoelectric point (calc.) |
7.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLVLDQLLL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |