Gene name |
SPAC12G12.12 |
Gene ID |
16/H12 |
Gene synonyms/obsolete |
|
Gene product |
NST UDP-galactose
transporter; no apparent Sc ortholog |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1087 |
ORF length (spliced) |
975 |
Entry clone length |
1087 |
No. of intron |
2 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match in both
ends |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC12G12.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTTTGCCAATTATTAT |
Rev primer name |
SPAC12G12.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACGATTCGGCGAGAGGAAAT |
Amino acid length |
324 |
Molecular weight |
35.7 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
6 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLQFLGFVLLI |
Localization (YFP) |
no expression clone
|
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
|