| Gene name |
SPAC24H6.10c |
| Gene ID |
17/D05 |
| Gene synonyms/obsolete |
|
| Gene product |
phospho-2-dehydro-3-deoxyheptonate aldolase;
shikimate pathway (1st step) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1107 |
| ORF length (spliced) |
|
| Entry clone length |
1107 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
237T:C / 543T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC24H6.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACAAGCACACTCCTCT |
| Rev primer name |
SPAC24H6.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGAGAACGACGAGAACGG |
| Amino acid length |
368 |
| Molecular weight |
39.7 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol>nucleus |
| Comments for localization |
no apparent
signal? |
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
Confocal
|