| Gene name |
SPAC227.18 |
| Gene ID |
17/D06 |
| Gene synonyms/obsolete |
lys3; SPAC2F7.01 |
| Gene product |
saccharopine
dehydrogenase [NAD+, L-lysine forming] |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1107 |
| ORF length (spliced) |
|
| Entry clone length |
1107 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC227.18.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTGCTCCTCATCTTTG |
| Rev primer name |
SPAC227.18.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGCTTTTGAACCATGGTT |
| Amino acid length |
368 |
| Molecular weight |
41.3 |
| Isoelectric point (calc.) |
8.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
a lot of moving
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |