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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC227.03c
Gene ID 17/E11
Gene synonyms/obsolete
Gene product mitochondrial carrier; involved in mitochondrial transport; unknown specificity
Entry clone Cloned
ORF length (unspliced) 1116
ORF length (spliced)
Entry clone length 1116
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC227.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTGATGATTCTCTAAA
Rev primer name SPAC227.03.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTCAGCCATTTGCGAACA
Amino acid length 371
Molecular weight 41.3
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain 4
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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