Gene name |
SPCC126.05c |
Gene ID |
17/E12 |
Gene synonyms/obsolete |
|
Gene product |
mitochondrial
ribosomal protein L30 |
Entry clone |
Cloned |
ORF length (unspliced) |
1116 |
ORF length (spliced) |
807 |
Entry clone length |
1116 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
146T:C / 206A:G /
475T:C / 967T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC126.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTACTTGAAGAGAAATAT |
Rev primer name |
SPCC126.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAAACGATCGGAAAGTATG |
Amino acid length |
268 |
Molecular weight |
31 |
Isoelectric point (calc.) |
9.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRSLDKSLYL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |