| Gene name |
SPAC57A7.09 |
| Gene ID |
17/F01 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; zinc finger protein; zf-C3HC4 type (RING
finger); ubiquitin ligase (E3); protease associated (PA)
domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1119 |
| ORF length (spliced) |
|
| Entry clone length |
1119 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC57A7.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGTATTATCAAATAAT |
| Rev primer name |
SPAC57A7.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGAATGGCTTGGGTGGTGGA |
| Amino acid length |
372 |
| Molecular weight |
42.5 |
| Isoelectric point (calc.) |
5.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLEVIALDL/LITVQALAI |
| Localization (YFP) |
Golgi; vacuole |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |