Gene name |
SPAC22F8.02c |
Gene ID |
17/F02 |
Gene synonyms/obsolete |
|
Gene product |
PvGal biosynthesis
protein Pvg5; predicted N-terminal signal sequence; involved
in cell wall biosynthesis; deletion mutant galactomannans
deficient for pyruvylated
galactose-beta-1,3-galactose-alpha-1,2-pyruvate (PvGal); no
apparent orthologs; Sp specific families |
Entry clone |
Cloned |
ORF length (unspliced) |
1119 |
ORF length (spliced) |
|
Entry clone length |
1119 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
288A:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC22F8.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGCCTTCCATTACGGAT |
Rev primer name |
SPAC22F8.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGTTGCTTCCATTGTTCA |
Amino acid length |
372 |
Molecular weight |
43 |
Isoelectric point (calc.) |
7.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRLFLFGSLIL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|