| Gene name |
SPBC56F2.10c |
| Gene ID |
17/F09 |
| Gene synonyms/obsolete |
|
| Gene product |
dolichyl-phosphate
beta-glucosyltransferase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1124 |
| ORF length (spliced) |
969 |
| Entry clone length |
1124 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC56F2.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAGTATACATCGTTTT |
| Rev primer name |
SPBC56F2.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTAATACGCTTAGCCGAG |
| Amino acid length |
322 |
| Molecular weight |
36.7 |
| Isoelectric point (calc.) |
8.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAGFILLFL/LMHCFHKLLQI/LARFFGLPI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |