| Gene name |
SPCC1442.12 |
| Gene ID |
17/F08 |
| Gene synonyms/obsolete |
|
| Gene product |
CDP-diacylglycerol--serine
O-phosphatidyltransferase; involved in phosphatidylserine
biosynthesis; CDP-diacylglycerol-serine O-
phosphatidyltransferase activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1123 |
| ORF length (spliced) |
753 |
| Entry clone length |
1123 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
189A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1442.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTAGATCACGAGTTTC |
| Rev primer name |
SPCC1442.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACATATTTGAGCGAATGT |
| Amino acid length |
250 |
| Molecular weight |
27.7 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
3 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytoplasmic dots;
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol; cytoplasmic dots |
| Microscope used for
observation |
Confocal |