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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G6.04c
Gene ID 18/B06
Gene synonyms/obsolete cpp1
Gene product protein farnesyltransferase (beta subunit); involved in protein prenylation; non-essential; mutant is hypersensitive to canavanine
Entry clone Cloned#
ORF length (unspliced) 1149
ORF length (spliced)
Entry clone length 1149
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC17G6.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGAATTATCAGAAAC
Rev primer name SPAC17G6.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGAATTTTGATGATTCTTGT
Amino acid length 382
Molecular weight 42.1
Isoelectric point (calc.) 5.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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